Journal: iScience

Article Title: Spatiotemporal orchestration of macrophage activation trajectories by Vγ4 T cells during skin wound healing

doi: 10.1016/j.isci.2024.109545

Figure Lengend Snippet:

Article Snippet: Recombinant Mouse IL-4 Protein , Abclonal , Catalog: RP01161.

Techniques: Recombinant

Journal: ACS Applied Materials & Interfaces

Article Title: IL-4/Nanohydroxyapatite Codelivery in a Dual-Bionic Scaffold: Design and Immunomodulatory Endogenous Osteogenesis Mechanism

doi: 10.1021/acsami.5c23172

Figure Lengend Snippet: Schematic illustration of the bioactive PHS/SG@IL-4 dual-bionic scaffold: from bionic design to critical-sized calvarial regeneration. Inspired by the “soft-hard” integration structure of natural bone, we fabricated PCL/nHA composite filaments to construct gyroid bionic scaffolds through FDM 3D printing. Subsequently, SG hydrogel loaded with interleukin-4 (SG@IL-4) was infused into the scaffold pores using a vacuum-assisted technique. The resulting scaffold provides sustained release of IL-4 and bioactive nHA. These signals work synergistically to polarize macrophages toward the M2 phenotype and subsequently promote bone regeneration by modulating the PI3K-AKT and calcium signaling pathways.

Article Snippet: The SG/IL-4 hydrogel was prepared by homogeneously mixing the SG hydrogel precursor solution with recombinant mouse interleukin-4 (IL-4, Solarbio, China, cat. no. P00196) to achieve a final concentration of 1 μg/mL.

Techniques: Construct, Protein-Protein interactions

Journal: NPJ Parkinson's disease

Article Title: Altered expression of the immunoregulatory ligand-receptor pair CD200-CD200R1 in the brain of Parkinson's disease patients.

doi: 10.1038/s41531-022-00290-2

Figure Lengend Snippet: Fig. 2 CD200R1 expression in human microglia-like cells treated with inflammatory stimuli. mRNA expression of the four CD200R1 mRNA variants in microglia-like cells treated for 24 h with LPS (100 ng/mL) (a) or IL4 (50 ng/mL) (b) by qRT-PCR after gene-specific retrotranscription. GAPDH and RPS18 were used as reference genes. Individual points are represented and bars indicate the positions of the mean ± SEM of 3–4 independent experiments. *p < 0.05 and **p < 0.01; two-tailed paired Student’s t-test.

Article Snippet: Cells were treated with LPS from Escherichia coli (100 ng/mL; 026:B6; Sigma-Aldrich) or IL4 (50 ng/mL; recombinant mouse IL4 expressed in CHO cells, Creative BioMart, Shirley, NY, USA) and processed after 24 h. Control and treated cells were derived from the same donor.

Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test

Journal: Nature Communications

Article Title: CD163 interacts with TWEAK to regulate tissue regeneration after ischaemic injury

doi: 10.1038/ncomms8792

Figure Lengend Snippet: ( a ) TWEAK (100 ng ml −1 ) was added to VEGFR1, Fn14 or CD163-coated wells. Bound protein was measured by ELISA using an antibody to detect TWEAK. ( b ) Fn14 or CD163 were coated onto 96-well plates and incubated with increasing doses of untagged TWEAK. Bound protein was measured by ELISA. ( c ) Immunoblotting of MDEC stimulated with TWEAK at 200 ng ml −1 (which activates both canonical and non-canonical NF-κB signalling) and increasing doses of recombinant mouse sCD163 for the indicated proteins. ( d ) Immunoprecipitation of serum before and after femoral ligation using anti-TWEAK antibody with immunoblotting for CD163. (The figures show a representative blot with a total of four experiments conducted.) ( e ) Immunostaining for VE-cadherin and β-dystroglycan (red) in limbs of CD163 −/− mice treated for 14 days with saline (control), TWEAK administration (25 μg kg −1 day −1 administered via Alzet pump) alone or in combination with sCD163 administration (25 μg kg −1 day −1 administered by intraperitoneal injection) ( n =5 per group). The graphs show the number of VE-cadherin positive cells per myofibre and the average muscle fibre area per group. ( f ) Immunblotting of CD163 −/− limb after 14 days of TWEAK administration (25 μg kg −1 day −1 administered via Alzet pump) alone or in combination with sCD163 administration (25 μg kg −1 day −1 administered by intraperitoneal injection) ( n =5 per group). O.D., optical density. Bar graphs show quantitation of densitometry for phosphorylated to total p65, p52 and NICD. * P <0.05 versus other groups. For multiple group comparisons, we utilized a one-way ANOVA. If the variance ratio test ( F -test) was significant, a more detailed post hoc analysis of differences between groups was made using a Tukey–Kramer honest significance difference test. For binding experiments, average of at least four well per group shown with each experiment repeated at least two times with representative results shown.

Article Snippet: For experiments shown in , mouse recombinant TWEAK (untagged) was obtained from Kingfisher Biotech.

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Western Blot, Recombinant, Immunoprecipitation, Ligation, Immunostaining, Saline, Control, Injection, Quantitation Assay, Binding Assay